7 extractive question-and-answer pairs built from Assessment of genetic purity of F1 progeny in Indian mustard (Brassica juncea L.) using microsatellite markers | Annals of Agricultural Research, published by epubs.icar.org.in. Source-evidenced topics include genetic purity, f1 progeny, indian mustard, brassica juncea, microsatellite markers, ssr primers, hybrid plants, parental lines. Every answer is a verbatim span of text the source prints, and each row carries the passage it sits in, its offset in that passage, the source quote, the page and the location in the document, so any row can be checked against the original. Question types in the export: 3 factual, 2 definition, 1 list, 1 relationship. 4 of the 7 pairs (57.1%) ask explanatory questions; the remaining 3 are factual or table-based questions. 100.00% of rows pass the corpus quality gate.
This dataset includes seven extractive question-and-answer pairs from research on genetic purity assessment in Indian mustard (Brassica juncea L.) using microsatellite markers. The study aimed to identify true hybrids in F1 progeny derived from a cross between RSPR-01 and Heera cultivars. Molecular characterization relied on single sequence repeat (SSR) markers, with 25 random primer pairs screened, of which seven were polymorphic. Specific SSR markers were identified to distinguish hybrid plants from parental lines and support breeding programs.
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df.head()First 7 of 7 rows
| question | answer | context | answer_start | question_type | knowledge_quality_score | source_document | source_quote | source_page | source_location | confidence | validation_status |
|---|---|---|---|---|---|---|---|---|---|---|---|
| What is one application of the identified specific SSR/microsatellite markers? | identify the genetic purity of hybrids | The identified specific SSR/microsatellite markers can be utilized to identify the genetic purity of hybrids and it also help in improvement of any of the parent cultivars using pedigree/backcross breeding programmes in Indian mustard. | 70 | definition | 1.000 | https://epubs.icar.org.in/index.php/AAR/article/view/127481 | The identified specific SSR/microsatellite markers can be utilized to identify the genetic purity of hybrids and it also help in improvement of any of the parent cultivars using pedigree/backcross breeding programmes in Indian mustard. | p[0] | 0.700 | valid | |
| What was the basis for molecular characterization of parents and F1 progeny? | amplification pattern of single sequence repeats (SSR) markers | The current research work was undertaken to identify the true to type hybrids in the F1 progeny derived from cross of RSPR-01 and Heera cultivars. Molecular characterization of parents and F1 progeny was based on the amplification pattern of single sequence repeats (SSR) markers. A total of 25 random SSR primer pairs were used for screening of parental genotypes, out of which 7 primers were found polymorphic. Three SSR primers BR_ A04_9627743, BR_ A05_25290881 and BR_ A09_3888866 were selected as the specific markers which enabled to distinguish hybrid plants from their parental lines. Six out of the seven plants confirmed their true hybrid nature as both the parental alleles were expressed. | 217 | definition | 1.000 | https://epubs.icar.org.in/index.php/AAR/article/view/127481 | Molecular characterization of parents and F1 progeny was based on the amplification pattern of single sequence repeats (SSR) markers. | p[0] | 0.700 | valid | |
| How can the identified SSR markers assist in breeding programmes? | improvement of any of the parent cultivars using pedigree/backcross breeding programmes | The identified specific SSR/microsatellite markers can be utilized to identify the genetic purity of hybrids and it also help in improvement of any of the parent cultivars using pedigree/backcross breeding programmes in Indian mustard. | 129 | relationship | 0.970 | https://epubs.icar.org.in/index.php/AAR/article/view/127481 | The identified specific SSR/microsatellite markers can be utilized to identify the genetic purity of hybrids and it also help in improvement of any of the parent cultivars using pedigree/backcross breeding programmes in Indian mustard. | p[0] | 0.700 | valid | |
| Which SSR primers were selected as specific markers to distinguish hybrid plants from parental lines? | BR_ A04_9627743, BR_ A05_25290881 and BR_ A09_3888866 | The current research work was undertaken to identify the true to type hybrids in the F1 progeny derived from cross of RSPR-01 and Heera cultivars. Molecular characterization of parents and F1 progeny was based on the amplification pattern of single sequence repeats (SSR) markers. A total of 25 random SSR primer pairs were used for screening of parental genotypes, out of which 7 primers were found polymorphic. Three SSR primers BR_ A04_9627743, BR_ A05_25290881 and BR_ A09_3888866 were selected as the specific markers which enabled to distinguish hybrid plants from their parental lines. Six out of the seven plants confirmed their true hybrid nature as both the parental alleles were expressed. | 431 | list | 0.820 | https://epubs.icar.org.in/index.php/AAR/article/view/127481 | Three SSR primers BR_ A04_9627743, BR_ A05_25290881 and BR_ A09_3888866 were selected as the specific markers which enabled to distinguish hybrid plants from their parental lines. | p[0] | 0.700 | valid | |
| Which cultivars were crossed to derive the F1 progeny in the research? | RSPR-01 and Heera cultivars | The current research work was undertaken to identify the true to type hybrids in the F1 progeny derived from cross of RSPR-01 and Heera cultivars. Molecular characterization of parents and F1 progeny was based on the amplification pattern of single sequence repeats (SSR) markers. A total of 25 random SSR primer pairs were used for screening of parental genotypes, out of which 7 primers were found polymorphic. Three SSR primers BR_ A04_9627743, BR_ A05_25290881 and BR_ A09_3888866 were selected as the specific markers which enabled to distinguish hybrid plants from their parental lines. Six out of the seven plants confirmed their true hybrid nature as both the parental alleles were expressed. | 118 | factual | 0.490 | https://epubs.icar.org.in/index.php/AAR/article/view/127481 | The current research work was undertaken to identify the true to type hybrids in the F1 progeny derived from cross of RSPR-01 and Heera cultivars. | p[0] | 0.700 | valid | |
| How many random SSR primer pairs were used for screening of parental genotypes, out of which 7 primers were found polymorphic? | 25 | Molecular characterization of parents and F1 progeny was based on the amplification pattern of single sequence repeats (SSR) markers. A total of 25 random SSR primer pairs were used for screening of parental genotypes, out of which 7 primers were found polymorphic. Three SSR primers BR_ A04_9627743, BR_ A05_25290881 and BR_ A09_3888866 were selected as the specific markers which enabled to distinguish hybrid plants from their parental lines. | 145 | factual | 0.370 | https://epubs.icar.org.in/index.php/AAR/article/view/127481 | A total of 25 random SSR primer pairs were used for screening of parental genotypes, out of which 7 primers were found polymorphic. | p[0] | 0.800 | valid | |
| How many primers were found polymorphic? | 7 | Molecular characterization of parents and F1 progeny was based on the amplification pattern of single sequence repeats (SSR) markers. A total of 25 random SSR primer pairs were used for screening of parental genotypes, out of which 7 primers were found polymorphic. Three SSR primers BR_ A04_9627743, BR_ A05_25290881 and BR_ A09_3888866 were selected as the specific markers which enabled to distinguish hybrid plants from their parental lines. | 232 | factual | 0.330 | https://epubs.icar.org.in/index.php/AAR/article/view/127481 | A total of 25 random SSR primer pairs were used for screening of parental genotypes, out of which 7 primers were found polymorphic. | p[0] | 0.800 | valid |
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