13 extractive question-and-answer pairs built from An improved DNA extraction method and standardisation of transmission studies of enation leaf curl virus in okra (Abelmoschus esculentus) | The Indian Journal of Agricultural Sciences, published by epubs.icar.org.in. Source-evidenced topics include dna extraction method, enation leaf curl virus, okra, abelmoschus esculentus, oelcuv, white fly, bemisia tabaci, malvaceae. Every answer is a verbatim span of text the source prints, and each row carries the passage it sits in, its offset in that passage, the source quote, the page and the location in the document, so any row can be checked against the original. Question types in the export: 9 factual, 2 list, 2 relationship. 4 of the 13 pairs (30.8%) ask explanatory questions; the remaining 9 are factual or table-based questions. 100.00% of rows pass the corpus quality gate.
This dataset contains 13 question-and-answer pairs derived from a study on DNA extraction methods and virus transmission in okra (Abelmoschus esculentus). The research focuses on overcoming challenges in DNA isolation from Malvaceae plants, which are complicated by high mucilage content. It also examines the transmission efficiency of Okra enation leaf curl virus (OELCuV) via whiteflies. The dataset includes factual, list-based, and explanatory questions, with answers sourced directly from the study's text.
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import desidata
df = desidata.load("epubs-icar-org-in-58678ed3cdc8b32f71e9fa2c5e024f7c")
df.head()First 10 of 13 rows
| question | answer | context | answer_start | question_type | knowledge_quality_score | source_document | source_quote | source_page | source_location | confidence | validation_status |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Why is DNA isolation from Malvaceae plant families difficult? | high mucilage content, which binds to secondary metabolites and interferes with molecular studies | In India, okra (Abelmoschus esculentusL.) is severely affected by OELCuV (Okra enation leaf curl virus) which is influenced by vector (white fly,Bemisia tabaci), host and environment. DNA isolation from Malvaceae plant families is difficult due to high mucilage content, which binds to secondary metabolites and interferes with molecular studies. The study was carried out during 2023–2024 at ICAR-Indian Agricultural Research Institute, New Delhi to utilise a supplemented extraction buffer with 2.0 g of polyvinylpyrrolidone (PVP) per 50 mL to neutralise polyphenols. | 248 | relationship | 0.970 | https://epubs.icar.org.in/index.php/IJAgS/article/view/171478 | DNA isolation from Malvaceae plant families is difficult due to high mucilage content, which binds to secondary metabolites and interferes with molecular studies. | p[0] | 0.800 | valid | |
| What are the requirements for using material from The Indian Journal of Agricultural Sciences? | the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR | This work is licensed under aCreative Commons Attribution-NonCommercial-ShareAlike 4. International License. The copyright of the articles published inThe Indian Journal of Agricultural Sciencesis vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR. Print ISSN:0019-5022Online ISSN:2394-3319NAAS Rating (2026):6.7WoS Impact Factor (June 2026):0. Indexedin Elsevier Scopus, Clarivate Analytics ESCI, DOAJ.Plagiarism checkby iThenticate.The work is licensed underAttribution-NonCommercial-NoDerivatives 4. | 483 | relationship | 0.970 | https://epubs.icar.org.in/index.php/IJAgS/article/view/171478 | The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR. | ||||
| Which indexing services include The Indian Journal of Agricultural Sciences? | Elsevier Scopus, Clarivate Analytics ESCI, DOAJ | This work is licensed under aCreative Commons Attribution-NonCommercial-ShareAlike 4. International License. The copyright of the articles published inThe Indian Journal of Agricultural Sciencesis vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR. Print ISSN:0019-5022Online ISSN:2394-3319NAAS Rating (2026):6.7WoS Impact Factor (June 2026):0. Indexedin Elsevier Scopus, Clarivate Analytics ESCI, DOAJ.Plagiarism checkby iThenticate.The work is licensed underAttribution-NonCommercial-NoDerivatives 4. | 727 | list | 0.820 | https://epubs.icar.org.in/index.php/IJAgS/article/view/171478 | Indexedin Elsevier Scopus, Clarivate Analytics ESCI, DOAJ.Plagiarism checkby iThenticate.The work is licensed underAttribution-NonCommercial-NoDerivatives 4. | ||||
| What was used for phase separation and mucilage removal during DNA isolation? | chloroform: isoamyl alcohol (24:1) | okra (Abelmoschus esculentusL.) is severely affected by OELCuV (Okra enation leaf curl virus) which is influenced by vector (white fly,Bemisia tabaci), host and environment. DNA isolation from Malvaceae plant families is difficult due to high mucilage content, which binds to secondary metabolites and interferes with molecular studies. The study was carried out during 2023–2024 at ICAR-Indian Agricultural Research Institute, New Delhi to utilise a supplemented extraction buffer with 2.0 g of polyvinylpyrrolidone (PVP) per 50 mL to neutralise polyphenols. The samples were treated with chloroform: isoamyl alcohol (24:1) twice for effective phase separation and mucilage removal. a washing step with 10 mM ammonium acetate was incorporated to eliminate residual polysaccharides and proteins, enhancing DNA purity. | 590 | list | 0.740 | https://epubs.icar.org.in/index.php/IJAgS/article/view/171478 | The samples were treated with chloroform: isoamyl alcohol (24:1) twice for effective phase separation and mucilage removal. | p[0] | 0.700 | ||
| What virus severely affects okra (Abelmoschus esculentus L.)? | OELCuV (Okra enation leaf curl virus) | okra (Abelmoschus esculentusL.) is severely affected by OELCuV (Okra enation leaf curl virus) which is influenced by vector (white fly,Bemisia tabaci), host and environment. DNA isolation from Malvaceae plant families is difficult due to high mucilage content, which binds to secondary metabolites and interferes with molecular studies. The study was carried out during 2023–2024 at ICAR-Indian Agricultural Research Institute, New Delhi to utilise a supplemented extraction buffer with 2.0 g of polyvinylpyrrolidone (PVP) per 50 mL to neutralise polyphenols. The samples were treated with chloroform: isoamyl alcohol (24:1) twice for effective phase separation and mucilage removal. a washing step with 10 mM ammonium acetate was incorporated to eliminate residual polysaccharides and proteins, enhancing DNA purity. | 56 | factual | 0.570 | https://epubs.icar.org.in/index.php/IJAgS/article/view/171478 | okra (Abelmoschus esculentusL.) is severely affected by OELCuV (Okra enation leaf curl virus) which is influenced by vector (white fly,Bemisia tabaci), host and environment. | p[0] | 0.700 | ||
| What was added to the extraction buffer to counteract polyphenols? | 2.0 g of polyvinylpyrrolidone (PVP) per 50 mL | okra (Abelmoschus esculentusL.) is severely affected by OELCuV (Okra enation leaf curl virus) which is influenced by vector (white fly,Bemisia tabaci), host and environment. DNA isolation from Malvaceae plant families is difficult due to high mucilage content, which binds to secondary metabolites and interferes with molecular studies. The study was carried out during 2023–2024 at ICAR-Indian Agricultural Research Institute, New Delhi to utilise a supplemented extraction buffer with 2.0 g of polyvinylpyrrolidone (PVP) per 50 mL to neutralise polyphenols. The samples were treated with chloroform: isoamyl alcohol (24:1) twice for effective phase separation and mucilage removal. a washing step with 10 mM ammonium acetate was incorporated to eliminate residual polysaccharides and proteins, enhancing DNA purity. | 487 | factual | 0.570 | https://epubs.icar.org.in/index.php/IJAgS/article/view/171478 | The study was carried out during 2023–2024 at ICAR-Indian Agricultural Research Institute, New Delhi to utilise a supplemented extraction buffer with 2.0 g of polyvinylpyrrolidone (PVP) per 50 mL to neutralise polyphenols. | ||||
| Who holds the copyright for articles published in The Indian Journal of Agricultural Sciences? | the Indian Council of Agricultural Research | This work is licensed under aCreative Commons Attribution-NonCommercial-ShareAlike 4. International License. The copyright of the articles published inThe Indian Journal of Agricultural Sciencesis vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR. Print ISSN:0019-5022Online ISSN:2394-3319NAAS Rating (2026):6.7WoS Impact Factor (June 2026):0. Indexedin Elsevier Scopus, Clarivate Analytics ESCI, DOAJ.Plagiarism checkby iThenticate.The work is licensed underAttribution-NonCommercial-NoDerivatives 4. | 209 | factual | 0.570 | https://epubs.icar.org.in/index.php/IJAgS/article/view/171478 | The copyright of the articles published inThe Indian Journal of Agricultural Sciencesis vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. | ||||
| How many h after acquisition and inoculation access periods using 12 whiteflies/seedling? | 24 | Virus acquisition and inoculation required minimum access periods of 45 min, with transmission efficiency increasing over time and vector density. Maximum transmission (100%) was observed at 24 h after acquisition and inoculation access periods using 12 whiteflies/seedling. Younger seedlings (10–12 days old) exhibited the highest susceptibility, while no transmission occurred in plants older than 45 days. | 191 | factual | 0.370 | https://epubs.icar.org.in/index.php/IJAgS/article/view/171478 | Maximum transmission (100%) was observed at 24 h after acquisition and inoculation access periods using 12 whiteflies/seedling. | p[0] | 0.800 | valid | |
| How many mM ammonium acetate was incorporated to eliminate residual polysaccharides and proteins, enhancing DNA purity? | 10 | Additionally, a washing step with 10 mM ammonium acetate was incorporated to eliminate residual polysaccharides and proteins, enhancing DNA purity. This protocol yielded high quality DNA with an average concentration of 1501.35 ng/μL and purity ratios of A260/A280 = 1.82 and A260/A230 = 1.78, outperforming commercial kits and conventional methods of DNA isolation. | 34 | factual | 0.370 | https://epubs.icar.org.in/index.php/IJAgS/article/view/171478 | a washing step with 10 mM ammonium acetate was incorporated to eliminate residual polysaccharides and proteins, enhancing DNA purity. | p[0] | 0.800 | valid | |
| How many min, with transmission efficiency increasing over time and vector density? | 45 | Using a controlled whitefly culture, the study assessedBegomovirus abelmoschusenationtransmission efficiency across various okra seedling growth stages. Virus acquisition and inoculation required minimum access periods of 45 min, with transmission efficiency increasing over time and vector density. Maximum transmission (100%) was observed at 24 h after acquisition and inoculation access periods using 12 whiteflies/seedling. | 222 | factual | 0.370 | https://epubs.icar.org.in/index.php/IJAgS/article/view/171478 | Virus acquisition and inoculation required minimum access periods of 45 min, with transmission efficiency increasing over time and vector density. | p[0] | 0.800 | valid |
Read straight from the file — download or use the API URL for the full dataset.
| p[31] |
| 0.700 |
| valid |
| p[31] |
| 0.700 |
| valid |
| valid |
| valid |
| p[0] |
| 0.700 |
| valid |
| p[31] |
| 0.700 |
| valid |